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PROMAX™ Acid protease

时间: 2022-09-29
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Overview

Acid proteasederived from strains of Aspergillus Niger, under the condition of low pH,hydrolyze protein effectively, used in animal feed, feed-additives and feedcompositions as exogenous enzymes in the feed industry, or treat vegetableproteins.


Action Mechanism

Acid protease contains aspartate inits active center, which can widely cut the peptide bond in amino acid, preferto cut the peptide bond between aromatic or hydrophobic amino acid residues atboth ends, especially the peptide bond between aromatic amino acid and otheramino acid, producing small peptides and amino acids.


Product Characteristics

1. Product specifications: 10,000 u/g~50,000u/g. powdered solid, enzyme fineness (0.4 mm standard screen) ≥80%

2. Appearance characteristics: Brown powder

3. Carrier: sweet potato flour

4. Product standards: execute the People'sRepublic of China national standard GB/T23527-2009.


Enzyme activity definition

One acidprotease activity unit is defined as the amount of enzyme which liberates 1μgtyrosine per minute from casein at 40℃ and pH 3.0.

Optimum pH

Acid protease’s optimum reaction pH is low, and basically coherent with thedigestive system of livestock and poultry. Make up for a lack of livestock andpoultry intestinal secretion of endogenous protease, improve proteindigestibility, reduce metabolic diarrhea when used in feed additive. Theeffective pH range is 2.0 to 6.0, the optimum pH is 4.0


Temperature curve

When temperature is lower than 30 ℃ or higher than 45 ℃, the relative enzyme activity decreased obviously. The effective temperature range and the optimum temperature is 35~40℃,40℃,respectively(see fig.2).


PROMAX™ Acid protease


The Effect of Metal Ions

Metal ion concentration has different effect on enzyme activity, the activation effect is often low concentration, and high concentration of inhibition. Figure3 shows that under the concentration of 5.0 mmol/L, Mn2+and Cu2+have a strong activated effect on acid protease produced by Aspergillus Niger, 1.5-fold and 1.2-fold of the control, respectively. Fe3+ show obvious inhibition of this enzyme. Fe2+, Ag+, Zn2+, Ca2+,K+, and Mg2+ have some inhibitory effect of acid protease.

 

PROMAX™ Acid protease


Recommended Dosage of Acid Protease

PROMAX™ Acid protease

The recommend dosage is based on 50,000 U/g product


Package

20 kg per bag


Usage and Storage

1.Keep away from the moisture and avoid storing in a high-temperature environment

2.To avoid the sun and the rain.

3.Keep the warehouse clean, cool and dry.

4.Do not put it in a carbon steel tank for a long time.


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